The air-liquid interface and use of primary cell cultures are important to recapitulate the transcriptional profile of in vivo airway epithelia.
- PubMed - NCBI
The NCBI web site requires JavaScript to function.
FormatSummarySummary (text)AbstractAbstract (text)MEDLINEXMLPMID ListChoose DestinationFileClipboardCollectionsE-mailOrderMy BibliographyCitation managerFormatSummary (text)Abstract (text)MEDLINEXMLPMID ListCSVCreate File1 selected item: FormatSummarySummary (text)AbstractAbstract (text)MEDLINEXMLPMID ListMeSH and Other DataE-mailSubjectAdditional textE-mailAdd to ClipboardAdd to CollectionsOrder articlesAdd to My BibliographyGenerate a file for use with external citation management software.Create File
):L25-31. doi: 10.1152/ajplung.. Epub
2010 Oct 22.The air-liquid interface and use of primary cell cultures are important to recapitulate the transcriptional profile of in vivo airway epithelia.1, , , , , , , , .1Department of Internal Medicine, Univ. of Iowa, Iowa City, 52242, USA.AbstractOrganotypic cultures of primary human airway epithelial cells have been used to investigate the morphology, ion and fluid transport, innate immunity, transcytosis, infection, inflammation, signaling, cilia, and repair functions of this complex tissue. However, we do not know how closely these cultures resemble the airway surface epithelium in vivo. In this study, we examined the genome-wide expression profile of tracheal and bronchial human airway epithelia in vivo and compared it with the expression profile of primary cultures of human airway epithelia grown at the air-liquid interface. For comparison, we also investigated the expression profile of Calu-3 cells grown at the air-liquid interface and primary cultures of human airway epithelia submerged in nutrient media. We found that the transcriptional profile of differentiated primary cultures grown at the air-liquid interface most closely resembles that of in vivo airway epithelia, suggesting that the use of primary cultures and the presence of an air-liquid interface are important to recapitulate airway epithelia biology. We describe a high level of similarity between cells of tracheal and bronchial origin within and between different human donors, which suggests a very robust expression profile that is specific to airway cells.PMID:
[PubMed - indexed for MEDLINE] PMCID: PMC3023285 Unsupervised hierarchical clustering of airway epithelial cells. RNA was extracted from samples of Calu-3 cells (n = 12; red box), in vitro airway epithelia submerged in nutrient media (n = 6; yellow box), in vitro differentiated airway epithelia (n = 16; blue box), and in vivo airway epithelia (n = 16; green box). Biotinylated cRNA was synthesized following the manufacturer's protocol and then hybridized to a custom GeneChip Human Airway Array (HsA Affymetrix). Normalized data were analyzed in GenePattern (http://www.broadinstitute.org/cancer/software/genepattern/) using the “HierarchicalClustering” module with Pearson correlation as distance measure and pairwise complete linkage as clustering method.Am J Physiol Lung Cell Mol Physiol. ):L25-L31.One-way ANOVA comparing tissue from bronchus and trachea. Normalized microarray expression data from tracheal and bronchial samples of in vivo airway epithelia (A) and in vitro differentiated airway epithelia (B) were analyzed using 1-way ANOVA. Average probe fold change levels between bronchial and tracheal samples with their respective P values are displayed as volcano plots. FDR, false discovery rate.Am J Physiol Lung Cell Mol Physiol. ):L25-L31.Unsupervised hierarchical clustering of in vivo airway epithelia and in vitro differentiated airway epithelia. Normalized microarray expression data from in vitro differentiated airway epithelia (n = 16; blue box) and in vivo airway epithelia (n = 16; red box) were analyzed in GenePattern using the HierarchicalClustering module with Pearson correlation as distance measure and pairwise complete linkage as clustering method. Labels denote donor (#) and tracheal (T) or bronchial (B) source of tissue.Am J Physiol Lung Cell Mol Physiol. ):L25-L31.ANOVA for expression data from in vivo airway epithelia and in vitro differentiated airway epithelia. A: normalized microarray expression data from samples of in vivo airway epithelia and in vitro differentiated airway epithelia were analyzed using 1-way ANOVA. Average probe fold change levels between in vitro and in vivo samples with their respective P values are displayed as a volcano plot. Genes differentially expressed at a log2 fold change level & 3 with an FDR & 1% are displayed as a heat map in B. Labels denote donor (#) and tracheal (T) or bronchial (B) source of tissue.Am J Physiol Lung Cell Mol Physiol. ):L25-L31.Publication TypesMeSH TermsSubstancesGrant SupportFull Text SourcesOther Literature Sources
Supplemental Content
External link. Please review our .sihan-hi的美妆日志　
用户昵称：
用户年龄：
TA的皮肤：
混合性皮肤　　
TA的发质：
使用心得：
共篇，条精华
共个分类，共款产品
加入品牌：
TA在用的产品：
TA的化妆品心得(共7篇心得，0篇精华)
&&所有心得
现在天气很干燥 加上自己又是干性皮肤 稍不注意就会起皮皮啊 好烦人的 用过一些保湿类的化妆品 刚用的时候感觉都不错 真要长期用了 就总觉得欠
已有<font id="cbAvail人推荐已有1人回复
早就看到别的试用网看到商家有搞这个活动 只是他们是要凭短信去专柜领取 我们的小县城根本就没有羽西专柜 呜呜。。。。难道我们小县城的人就没
已有<font id="cbAvail人推荐已有1人回复
使用外观:虽然只是试用装 但是跟其他商家相比包装质地要很多
拿在手里感觉很厚实
使用感受:因为整天面对电脑 皮
已有<font id="cbAvail人推荐已有0人回复
没听过这个牌子 但是见包装不错 想试一下 看看这个牌子适不适合自己
已有<font id="cbAvail人推荐已有3人回复
早就在官网上看到这款面膜 一直以来都注重晚上皮肤的护理工作 这款当然不想错过啦 但是银子有限 嘿嘿 先试用一下 试试效果
已有<font id="cbAvail人推荐已有2人回复
光看瓶子 就很上档次的感觉很吸引人啊 嘿嘿 土一个先 不知效果好不好 好希望能得到这款啊
已有<font id="cbAvail人推荐已有2人回复
先发下牢骚 嘿嘿 当时没有工作姐姐大婚 作为&#31036;物送给她的
真的是狠狠心才拿下来的
不过效果也是很显著呢 排除价&#26684;因
已有<font id="cbAvail人推荐已有3人回复
天生就长的黑 汗一个先 ╮(╯▽╰)╭ 俗话说一白遮百丑 所以总是想自己白白嫩嫩的，嘻嘻 以前用过挺多美容产品 有的刚用的时候效果不错 慢慢的
已有<font id="cbAvail人推荐已有2人回复
谁刚点评了什么
刚刚点评：御泥坊又有新品面膜了，而且是全球首发款泥浆面膜
刚刚点评：近期花了太多心思在美白上，彩妆方面入手不多，于
刚刚点评：炎热的夏天来了，防晒必不可少~太阳帽、眼镜、还
刚刚点评：温和的保湿型洁面乳，质感细腻，丝般幼滑，蕴含乳
刚刚点评：玫瑰保湿喷雾蕴含天然玫瑰花水，即时为冬季干燥肌
每周我们会定期将YOKA电子刊物发送您的邮箱中
若需退订，请生意宝检测通，检测认证一站式平台！
您所在的位置： &
& 世联博研签约瑞士Cellectricon Cellaxess ACE原位贴壁原代和iPSC衍生细胞电穿孔电转染仪
订阅我们的资讯
关注我们的新浪微博
　中国的测试测量市场正呈现出一种“变化”的格局。随着新技术及应用的不断涌现，测试测量...[]
据由日本大阪大学教授丝崎秀夫主持开发的新型液体快速检测仪，日前结束了在东京成田机场国际线的试用期...[]
GB 《食品中农药最大残留限量》被称为“史上最严”农残限量标准。...[]
&&上海市邯郸路99号上海材料研究所
&&上海材料研究所检测中心，上海市邯郸路99号
世联博研签约瑞士Cellectricon Cellaxess ACE原位贴壁原代和iPSC衍生细胞电穿孔电转染仪
来源：世联博研（北京）科技有限公司 时间：
电穿孔(Electroporation ), 也叫电转染。是功能强大的将核酸、蛋白及其它分子导入多种细胞的高效技术。通过高强度的电场作用，瞬时提高细胞膜的通透性，从而吸收周围介质中的外源分子。这种技术可以将核苷酸、DNA 与RNA、蛋白、糖类、染料及病毒颗粒等导入原核和真核细胞内。电转化相对其它物理和化学转化方法，是一种有价值和有效的替代方法
世联博研北京科技有限公司是瑞典Cellectricon授权代理商,签约代理
以下产品：
1、Dynaflow Resolve,Dynaflow离子通道受体、安全药理测、试药物筛查和最优化工具
2、cellaxess ACE单一电极电穿孔电转染系统,Cellaxess? ACE System
3、Cellaxess Elektra Discovery Platform(药物发现平台)
欢迎致电全国免费客服热线：400-650-8506 资信、索取产品详细资料
Cellaxess ACE原位贴壁原代细胞和iPSC衍生细胞电穿孔电转染仪，
(Cellectricon Cellaxess ACE Adherent Cell Electroporation,In-situ Transfection of Adherent Primary and iPSC-derived Cells
该系统显著特点是：
&O全面的灵活性 & 适用于任何类型的细胞，任何基材(Perfectly retained cell function and morphology)
&O完美保留了细胞的功能和形态(Complete flexibility & any cell type, any substrate)
&O高效且易于使用 (Efficient and easy to use)
In-situ transfection of adherent primary and iPSC? derived cells at any developmental stage
原位转染任何发展阶段的贴壁原代和iPSC衍生细胞
Cellaxess electroporation gives you superior and dependable in-plate transfection as low voltages and no cell processing are required.
With Cellaxess ACE, cells can be transfected at any stage of development with no impact on cell health or morphology. Cellaxess ACE enables in-situ transfection of any primary
and iPSC-derived cell type in its adherent state. The system is very easy to use and provides significant time savings.
Gene transfer
siRNA delivery
Small molecule, protein, peptide and probe delivery
&OPerfectly retained cell function and morphology
&ONo impact on cell viability
&OLow cell and substrate consumption
&OHighly efficient, providing significant time savings
&ONo cell processing
&OProtocols for a wide range of adherent cells
Transfection with stunning results
Complete system or plug & play module
Superb electroporation has never been easier! Cellaxess ACE is available either as a complete system including pulse generator, or as a stand-alone module which can be combined with almost any pulse generator on the market. Accessing the Cellaxess electroporation platform is easy and highly cost-efficient.
Complete flexibility
Cellaxess ACE can be used with a wide range of cell culture formats - from single
dishes up to 96-well plates. The Cellaxess electrode is exchangeable, which ensures
experimental sterility and prevents the risk of cross contamination. Different
capillaries are available to enable high coverage electroporation in different cell
culture formats.
Ease of use
Cellaxess ACE is extremely easy to use, and offers huge time savings. A single
transfection reaction including preparation takes only a few minutes, an entire
24?well plate can be transfected in less than 15 minutes.
Technical Specifications:
Voltage & power requirements (Cellaxess ACE pulse generator only)
Voltage: 100-240 VAC, 50-60 Hz, CAT II
Power: 200 VA
Computer requirements (Cellaxess ACE pulse generator only)
Microsoft Windows 2000, XP, Vista or 7. Pentium P4 processor, 512 MB of RAM &
10 Mb of available hard-disk space. One available RS232 or USB 1.0 or 2.0 port.
Regulatory compliance
CE certified according to 89/336/EEC for Electromagnetic Compatibility (EC) and
Low-Voltage Directive (73/23/EEC) for product safety.
Cellaxess ACE System and
Associated Consumables
Product No: Product Name:
CXA100100 Cellaxess ACE System
CXA100200 Cellaxess ACE Module Kit
CXA200101 Cellaxess ACE capillary electrodes, small, 5-pack1
CXA200102 Cellaxess ACE capillary electrodes, medium, 5-pack2
CXA200103 Cellaxess ACE capillary electrodes, large, 5-pack3
1 Compatible with 384-well plates and lower density cell culture formats
2 Compatible with 96-well plates and lower density cell culture formats
3 Compatible with 48-well plates and lower density cell culture formats
Cellaxess& ACE
Cellaxess& ACE is a single electrode based electroporation system that can be used for any adherent cell type. Cellaxess& electroporation offers superior transfection quality because of minimal cell processing and low voltages required for electroporation.
Cellaxess& ACE can be used in any cell culture format, and is an excellent match with, high-content and confocal microscopy readouts. Cellaxess& ACE is available either as a complete system including pulse generator, or as a stand-alone module which can be combined with almost any pulse generator on the market.
Cellaxess& ACE enables transfection of any adherent cell type. Based on the same concept as Cellaxess& Elektra, the in-situ electroporation at low voltages results in transfections with unsurpassed efficiency and viability. Cellaxess& ACE is ideal for the transfection of challenging cell types, such as primary- and IPS derived neuronal cell types.
Cellaxess& ACE can be used in variety of cell culture formats, ranging from 96-well plates to dishes. It is also an excellent match with high-content and microscopy readouts.
Cellaxess& ACE is available either as a complete system including pulse generator, or as a stand-alone module which can be combined with almost any pulse generator on the market, making it a highly cost efficient solution and an easy way to assess electroporation performance for low volume electroporation.
Cellaxess& ACE has an exchangeable capillary, which ensures sterility in experiments, as well as zero risk of cross contamination. Furthermore, different capillaries can be utilized to accommodate for different cell culture formats.
Benefits优点
&OHighly efficient transfection of adherent cells such as primary- and IPS cells
高效的转染贴壁的细胞，例如原代和IPS细胞
&OPerfectly retained cell function and morphology
完美保留了细胞的功能和形态
&OIn-situ cell manipulation directly in cell culture dishes and micro plates
原位细胞操作，直接在细胞培养皿和微板
&OLittle/ no cell processing required
小/没有细胞处理
&OLow cell consumption
低细胞消耗量
&OHighly simplified experimental procedures
高度简化实验程序
Publications
Please click on the product buttons above right, to filter the publications to suit your requirements. Note: Cellaxess&HT was the previous name for Cellaxess& Elektra which has additional functionality for new applications.
Mitotic Motors Coregulate Microtubule Patterns in Axons and Dendrites
Shen Lin 1, Mei Liu 1,2, Olga I. Mozgova 1, Wenqian Yu 1, and Peter W. Baas 1,2
1) Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, Pennsylvania 19129, USA
2) Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, China
Published in: The Journal of Neuroscience, (2012) , 32(40):
High-Throughput Transfection of Differentiated Primary Neurons from Rat Forebrain
Shane Marine 1, Jamie Freeman 2, Antonella Riccio 2, Marie-Louise Axenborg 3, Johan Pihl 3, Robin Ketteler 2, and Sara Aspengren 3*
1) Department of Automated Biotechnology, Merck & Co., Inc., North Wales, PA, USA
2) MRC LMCB, University College of London, London, UK
3) *Cellectricon AB, M&lndal, Sweden, Email: sara.aspengren@cellectricon.se
Published in: Journal of Biomolecular Screening | March 8, 2012
Ef?ciency of Cellular Delivery of Antisense Peptide Nucleic Acid by Electroporation Depends on Charge and Electroporation Geometry
Mette Joergensen 1, Birgit Agerholm-Larsen 1,2, Peter E. Nielsen 3, and Julie Gehl 1.
1) Department of Oncology, Copenhagen University Hospital Herlev, Herlev, Denmark
2) Department of Neurology, Copenhagen University Hospital Glostrup, Glostrup, Denmark
3) Department of Cellular and Molecular Medicine, Health Science Faculty, University of Copenhagen, Copenhagen, Denmark
Published in: OLIGONUCLEOTIDES, Volume 21, Number 1, 2011
Cellaxess&HT cell-based assay and transfection lab
Sara Aspengren*, Michael Tokarz & Johan Pihl
*Cellectricon AB, Fl&jelbergsgatan 8C, SE-431 37 M&lndal, Sweden
Published in: NATURE METHODS | JUNE 2011
An NGF-responsive element targets myo-inositol monophosphatase-1 mRNA to sympathetic neuron axons
Catia Andreassi, Carola Zimmermann, Richard Mitter, Salvatore Fusco1, Serena De Vita, Adolfo Saiardi, & Antonella Riccio*
*Department of Neuroscience, Physiology and Pharmacology, University College London, London, UK
Published in: Nature Neuroscience 13, 291 - 301 (2010)
Transfection of chicken cerebellar granule neurons used to study glucocorticoid receptor regulation by nuclear receptor 4A (NR4A
Str&m BO, Aden P, Mathisen GH, L&mo J, Davanger S, Paulsen RE.
Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.
Published in: J Neurosci Methods. 2010 Oct 30;193(1):39-46. Epub 2010 Aug 19
FUNCTIONAL DIFFERENCES BETWEEN D1 AND D5 REVEALED BY HIGH RESOLUTION IMAGING ON LIVE NEURONS
M. KRUUSM&AGI, S. KUMAR, S. ZELENIN, H. BRISMAR, A. APERIA AND L. SCOTT*
*Department of Women&s and Children&s Health, Karolinska Institutet, Stockholm, Sweden
Published in: Neuroscience , 463&469
Cellaxess&HT: high-throughput transfection for genomewide RNAi
Johan Pihl*, Marie-Louise Johansson, Daniel Granfeldt, Michal Tokarz, Mattias Karlsson & Jon Sinclair
*Cellectricon AB, Fl&jelbergsgatan 8C, SE-431 37 M&lndal, Sweden
Published in: Nature Methods - 5, (2008)
Generation of Focused Electric Field Patterns at Dielectric Surfaces
Jessica Olofsson(1), Mikael Levin(2), Anette Str&mberg(2), Stephen G. Weber(3), Frida Rytts&n(2), and Owe Orwar(1)
1) Department of Chemistry and Bioscience, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden 2) Cellectricon AB, Fabriksgatan 7, SE-412 50 Gothenburg, Sweden 3) Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260
Published in: Anal. Chem. 67-4672
Fish Eggs Spawn a DNA Delivery Revolution & Caviar inspires a Berkeley bioengineer to take electroporation to the single-cell level
Laura lane
Published in: The Scientist 19 (13):34 (4 July 2005)
Electroporation and the Single Cell, New technologies increase the resolution of electroporation
Aileen Constans
Published in: The Scientist 18 (8):46 (26 April 2004)
Single-cell electroporation
Jessica Olofsson, Kerstin Nolkrantz, Frida Rytts&n, Bradley A. Lambie, Stephen G. Weber, Owe Orwar
Published in: Current Opinion in B 14(1), 29-34 (2003)
Functional Screening of Intracellular Proteins in Single Cells and in Patterned Cell Arrays Using Electroporation
Kerstin Nolkrantz, Cecilia Farre, K. Johan Hurtig, Petra Rylander, Owe Orwar
Published in: Anal. Chem. 00-4305
Electroporation of Single Cells and Tissues with an Electrolyte-filled Capillary
K. Nolkrantz, C. Farre, A. Brederlau, R.I.D. Karlsson, C. Brennan, P.S. Eriksson, S.G. Weber, M. Sandberg, O. Orwar
Published in: Anal. Chem., Sept 15, 73(18),
A method for bidirectional solution exchange & &Liquid bullet& applications of acetylcholine to a7 nicotinic receptors
Nikolai Fedorov (1), Lisa Benson (1), John D. Graef (1), Jeremy Hyman (1), Jill Sollenberger (1), Fredrik Pettersson (2), Patrick M. Lippiello (1), Merouane Bencherif (1)
1. Targacept, Inc., Preclinical Research Department, 200 East First Street, Suite #300, Winston Salem, NC 27101, USA
2. Veprox AB, H&ngpilsgatan 6, SE-426 77 V&stra Fr&lunda, Sweden
Published in: Journal of Neuroscience Methods, Volume 206, Issue 1, 30 April 2012, Pages 23&33
Alternatively spliced domains interact to regulate BK potassium channel gating
Brandon E. Johnson (a), Dominique A. Glausern (a), Elise S. Dan-Glauser (b), D. Brent Halling (c), Richard W. Aldrich (c) and Miriam B. Goodman (a).
a) Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305;
b) Department of Psychology, Stanford University, Stanford, CA 94305
c) Section of Neurobiology, Center for Learning and Memory, University of Texas, Austin, TX 78712
Published in: PNAS December 20, 2011 vol. 108 no. 51
Arrangement of Kv1 alpha subunits dictates sensitivity to tetraethylammonium
Al-Sabi A, Shamotienko O, Dhochartaigh SN, Muniyappa N, Le Berre M, Shaban H, Wang J, Sack JT, Dolly JO.
International Centre for Neurotherapeutics, Dublin City University, Dublin 9, Ireland.
Published in: J Gen Physiol. ):273-82.
Novel Alpha-7 Nicotinic Acetylcholine Receptor Agonists Containing a Urea Moiety: Identification and Characterization of the Potent, Selective, and Orally Efficacious Agonist 1-[6-(4-Fluorophenyl)pyridin-3-yl]-3-(4-piperidin-1-ylbutyl) Urea (SEN34625/WYE-103914)
Chiara Ghiron(1)
(1) Siena Biotech SpA, Strada del Petriccio e Belriguardo 35, 53100 Siena, Italy
(2) Chemical Sciences and Neuroscience Discovery Research, Wyeth Research, CN 8000, Princeton, New Jersey
Published in: J. Med. Chem., ), pp
DOI: 10.1021/jm901692q
Telithromycin blocks neuromuscular transmission and inhibits nAChR currents in vitro
Chang-Ning Liu, Chris J. Somps
Department of Investigative Toxicology, Drug Safety Research & Development, Pfizer Global R&D, Groton, CT 06340, USA
Published in: Toxicol Lett. 2010 May 4;194(3):66-9. Epub 2010 Feb 12.
MEC-2 and MEC-6 in the Caenorhabditis elegans Sensory Mechanotransduction Complex: Auxiliary Subunits that Enable Channel Activity
Austin L. Brown(1), Zhiwen Liao(2), and Miriam B. Goodman(1,2)
1) Biophysics Program and 2) Department of Molecular and Cellular Physiology, Stanford University, Stanford, CA 94305
Published in: J. Gen. Physiol., Jun : 605 - 616.
Differential Structure of Atrial and Ventricular KATP Atrial KATP Channels Require SUR1
Thomas P. Flagg(1); Harley T. Kurata(1); Ricard Masia(1); George Caputa(1); Mark A. Magnuson(2); David J. Lefer(3); William A. Coetzee(4); and Colin G. Nichols(1)
1) Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Mo; Department of Molecular Physiology 2) Biophysics, Vanderbilt University School of Medicine, Nashville, Tenn 3) Department of Medicine and Pathology, Albert Einstein College of Medicine, New Y 4) Department of Pediatrics, New York University School of Medicine.
Published in: Circulation Research. 2008 Published online before print October 30, 2008, doi: 10.1161/CIRCRESAHA.108.17818
Role of cAMP sensor Epac as a determinant of K-ATP channel ATP-sensitivity in human pancreatic beta cells and rat INS-1 cells
Guoxin Kang, Colin A Leech, Oleg G Chepurny, William A Coetzee, and George G Holz
New York University School of Medicine
Published in: Kang et al. J Physiol.2008; 0: jphysiol.v1
Gain-of-Function Mutations in the MEC-4 DEG/ENaC Sensory Mechanotransduction Channel Alter Gating and Drug Blockade
Austin L. Brown(1), Silvia M. Fernandez-Illescas(2), Zhiwen Liao2, and Miriam B. Goodman(1,2)
1) Biophysics Program and 2) Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, CA 94305
Published in: J. Gen. Physiol., Jan : 161 - 173.
Controlling Desensitized States in Ligand-Receptor Interaction Studies with Cyclic Scanning Patch-Clamp Protocols
Daniel Granfeldt(1), Jon Sinclair(2), Maria Millingen(1), Cecilia Farre(2), Per Lincoln(1) and Owe Orwar(1)
1) Department of Chemical and Biological Engineering, Physical Chemistry, Chalmers University of Technology, SE-412 96 G&teborg, Sweden 2) Cellectricon AB, Fabriksgatan 7, SE-412 50 G&teborg, Sweden
Published in: Anal. Chem.; 2006; ASAP A DOI: 10.1021/ac060812z
A Biohybrid Dynamic Random Access Memory
Jon Sinclair(1), Daniel Granfeldt(1), Johan Pihl(2), Maria Millingen(1), Per Lincoln(1), Cecilia Farre(2), Lena Peterson(3), and Owe Orwar(1)
1) Department of Chemistry and Bioscience and Microtechnology Centre, Chalmers University of Technology, SE-412 96 G&teborg, Sweden 2) Cellectricon AB, Fabriksgatan 7, SE-412 50 G&teborg, Sweden 3) Department of Signal and Systems, Chalmers University of Technology, SE-412 96 G&teborg, Sweden
Published in: J. Am. Chem. Soc.; 128(15), (doi:10.1021/ja0580993), (2006)
An isoflurane- and alcohol-insensitive mutant GABAA receptor 1 subunit with near normal apparent affinity for GABA: characterization in heterologous systems and production of knock-in mice.
Cecilia M. Borghese (1), David F. Werner (2), Norbert Topf (3), Nicole V. Baron (3), L. Ashley Henderson (4), Stephen L. Boehm II (5), Yuri A. Blednov (4), Abdallah Saad (6), Shuiping Dai (6), Robert A. Pearce (6), R. Adron Harris (1), Gregg E. Homanics (2), Neil L. Harrison(3)
1) University of Texas & Austin 2) University of Pittsburgh 3) Weill Medical College of Cornell University 4) University of Texas at Austin 5) State University of New York & Binghamton 6) University of Wisconsin & Madison
Published in: J. Pharmacol. Exp. Ther. 2006: jpet.106.
Blocking Characteristics of hERG, hNav1.5, and hKvLQT1/ hminK after Administration of the Novel Anti-Arrhythmic Compound AZD7009
Frida Persson, M.Sc.,Leif Carlsson, Ph.D., G&ran Duker, Ph.D., and Ingemar Jacobson, Ph.D.
AstraZeneca R&D M&lndal, M&lndal, Sweden
Published in: J Cardiovasc Electrophysiol, 16(3), 329-341,(2005)
Microfluidic device for creating gradients
Rajendrani Mukhopadhyay
Published in: Anal. Chem. A-P ); 245A-245A.
A chemical waveform synthesizer
Jessica Olofsson(1), Helen Bridle(1), Jon Sinclair(1), Daniel Granfeldt(1), Eskil Sahlin(2), and Owe Orwar(1)
1) Department of Chemistry and Bioscience and Microtechnology Centre, Chalmers University of Technology, SE-412 96 G&teborg, Sweden 2) Department of Chemistry, G&teborg University, SE-412 96 G&teborg, Sweden
Edited by Richard N. Zare, Stanford University, Stanford, CA
Published in: PNAS; 102(23), , (doi:10.1073/pnas.), (2005)
Microfluidic technologies in drug discovery
Johan Pihl, Mattias Karlsson, Daniel T. Chiu
Published in: Drug Discovery Today, Vol 10, Number 20, October 2005
Patch clamp electrophysiology steps up a gear
Derek J. Trezise, Assay & Compound Profiling, GlaxoSmithKline Research & Development
Published in: European Pharmaceutical review, Issue 2, 2005
Microfluidic Gradient Generating Device for Pharmacological Profiling
Johan Pihl(1), Jon Sinclair(1), Eskil Sahlin(2), Mattias Karlsson(3), Fredrik Petterson(3), Jessica Olofsson(1), and Owe Orwar(1)
1) Department of Chemistry and Bioscience, and Microtechnology Centre, Chalmers University of Technology, SE-412 96 G&teborg, Sweden 2) Department of Chemistry, G&teborg University, SE-412 96 G&teborg, Sweden 3) Cellectricon AB, Fabriksgatan 7 SE-412 50 G&teborg, Sweden
Published in: Analytical C 77(13), , (doi:10.1021/ac050218+), (2005)
Technology Feature: Cell biology, Maximizing return. Ion channels, stem cells and cell signalling are the focus of intense interest in both cell biology and drug discovery. Pete Moore takes a look at what&s on offer for the researcher.
Pete Moore
Published in: Nature, Dec 1, 439 (6817), p697-699 (2005). doi:10.b
Blocking characteristics of hKv1.5 and hKv4.3/hKChIP2.2 after administration of the novel antiarrhythmic compound AZD7009
Persson, Frida MSc; Carlsson, Leif PhD; Duker, Goran PhD; Ingemar Jacobson PhD
AstraZeneca R&D M&lndal, M&lndal, Sweden
Published in: Journal of Cardiovascular Pharmacology 46(1): 7-17. (2005).
Microfluidics and Patch-Clamp Based Sensors
Cecilia Farre, Mattias Karlsson, Jon Sinclair
Published in: Genetic Engineering News, Vol. 24, No 21, December 2004
A Microfluidics Approach to the Problem of Creating Separate Solution Environments Accessible from Macroscopic Volumes
Jessica Olofsson(1),Johan Pihl(1),Jon Sinclair(3),Eskil Sahlin(2), Mattias Karlsson(2), and Owe Orwar(1)
1)Department of Chemistry and Bioscience, and Microtechnology Centre, Chalmers University of Technology, SE-412 96 G&teborg, Sweden, 2) Cellectricon AB, Fabriksgatan 7 SE-412 50 G&teborg, Sweden, and 3) Department of Chemistry, Gothenburg University, SE-412 96 G&teborg, Sweden
Published in: Analytical C 76(17), 4968 - 4976, (doi:10.1021/ac035527j), (2004)
Stabilization of High-Resistance Seals in Patch-Clamp Recordings by Laminar Flow
Jon Sinclair, Jessica Olofsson, Johan Pihl, and Owe Orwar
Department of Physical Chemistry and Microtechnology Centre, Chalmers University of Technology, SE-412 96 G&teborg, Sweden
Published in: Analytical C 75(23), , (2003), (Technical Note)
Automated Electrophysiology: High Throughput of Art
Xiaobo Wang, Min Li
Published in: ASSAY and Drug Development Technologies. Oct 2003, Vol. 1, No. 5: 695-708
A Cell-Based Bar Code Reader for High-Throughput Screening of Ion Channel-Ligand Interactions
Jon Sinclair(1),Johan Pihl(1), Jessica Olofsson(1), Mattias Karlsson(1), Kent Jardemark(3), Daniel T. Chiu(2), and Owe Orwar(1)
1) Department of Physical Chemistry, and Microtechnology Centre, Chalmers University of Technology, SE-412 96 G&teborg, Sweden 2) Department of Chemistry, University of Washington, Seattle, Washington
3) Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden
Published in: Analytical C Dec 15, 74(24),
Screening of ion channel receptor agonists using capillary electrophoresis-patch clamp detection with resensitized detector cells
Cecilia Farre(1), Andreas Sj&berg(1), Kent Jardemark(1), Ingemar Jacobson(2), and Owe Orwar(1)
Department of Chemistry, G&teborg University, SE-412 96 G&teborg, Sweden, and AstraZeneca AB, SE-431 83 M&lndal, Sweden
Published in: Anal. Chem., Mar 15, 73(6), 01)
Life Science Technologies Microfluidics&Bringing New Things to Life Science
Jeffrey M. Perkel
Published in: AAAS/Science, 6 November 2008
Cellectricon: Insights from inside
Ludger Wess
Published in:
Selective introduction of antisense oligonucleotides into single adult CNS progenitor cells using electroporation demonstrates the requirement of STAT3 activation for CNTF-induced gliogenesis
&Aberg MA, Ryttsen F, Hellgren G, Lindell K, Rosengren LE, MacLennan AJ, Carlsson B, Orwar O, Eriksson PS.
Published in: Mol. Cell. Neurosci., Mar, 17(3), 426-43 (2001)
Chemical transformations in individual ultrasmall biomimetic containers
D. T. Chiu, C. F. Wilson, F. Rytts&n, A. Str&mberg, C. Farre, A. Karlsson, S. Nordholm, A. Gaggar, B. P. Modi, A. Moscho, R. A. Garza-L&pez, O. Orwar, R. N. Zare
Published in:
Electroinjection of colloid particles and biopolymers into single unilamellar liposomes and cells for bioanalytical applicatons
M. Karlsson, K. Nolkrantz, M. J. Davidson, A. Str&mberg, F. Rytts&n, B. &Akerman, O. Orwar
Published in: Anal. Chem., 72(23),
Manipulating the Genetic Identity and Biochemical Surface Properties of Individual Cells with Electric-Field-Induced Fusion
A. Str&mberg, F. Rytts&n, D. T. Chiu, M. Davidson, P. S. Eriksson, C. F. Wilson, O. Orwar, R. N. Zare
Published in: PNAS, 97(1), 7-11(2000)
Characterization of single-cell electroporation by using patch-clamp and fluorescence microscopy
Ryttsen F, Farre C, Brennan C, Weber SG, Nolkrantz K, Jardemark K, Chiu DT, Orwar O.
Published in: Biophys. J., O 79(4):
Altering the Biochemical State of Individual Cultured Cells and Organelles with Ultramicroelectrodes
J. A. Lundqvist, F. Sahlin, M. A. I. &Aberg, A. Str&mberg, P. S. Eriksson, O. Orwar
Published in: PNAS, 95,
Testimonials
&We have now a new electroporation procedure using the Cellaxess& system for hard-to-transfect neurons. Our obtained result on transient transfection of adherent primary neurons is published in: J Neurosci Methods. 2010&
Prof. Ragnhild E. Paulsen, Oslo University
&Thanks to the unique features of the Cellaxess& technology we are very pleased to have incorporated the technology into our research. Cellaxess& enables electroporation of hard-to-transfect cells and the results we obtained on striatum slice cultures were published in Neuroscience, 2009&
Dr. Lena Scott, Karolinska Institute
&The Cellaxess& technology enabled us to perform high-efficiency transfection of plasmid DNA and siRNAs in primary sympathetic neurons. The results obtained were published in Nature Neuroscience 2010.&
Dr. Catia Andreassi, MRC LMCB, University College London
　生物工程实验室是化工学院生物工程的专业实验室，包括生物工程综合实验室、发酵工程
东业德勤测试顾问（深圳）有限公司致力于打造中国最大、最专业的无损检测、工程咨询、
关注检测通微信公众号