请问western blot用什么设备做?_百度作业帮
请问western blot用什么设备做?
美国wealtec的V-GES加E-Blotter 或者要是用的起预制胶的话可以买mini V-GES就是垂直槽和湿转都有了 wealtec的半干也很厉害做7分钟快速半干只换个缓冲液就能做 进口品牌里性价比最高western blot_百度文库
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HIV ELISA/Western blot is a set of blood tests used to diagnose chronic infection with human immunodeficiency virus (HIV).A blood sample is drawn from a vein.No preparation is necessary.When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.Testing (screening) for HIV infection is done for many reasons, including for:Persons who want to be testedPersons in high-risk groups (men who have sex with men, injection drug users and their sexual partners, and commercial sex workers)Persons with certain conditions and infections (such as
pneumonia)Pregnant women, to help prevent them from passing the virus to the babyWhen a patient has an unusual infectionA negative test result is normal. But persons with early HIV infection (acute HIV infection or primary HIV infection) often have a negative test result.A positive result on the
screening test does not mean that the person has HIV infection. Certain conditions may lead to a false positive result, such as , , and lupus.A positive ELISA test is always followed by a Western blot test. A positive Western blot confirms an HIV infection. A negative Western blot test means the ELISA test was a false positive test. The Western blot test can also be unclear, in which case more testing is done.Negative tests do not rule out HIV infection. There is a period of time, called the window period, between HIV infection and the appearance of anti-HIV . During this period, antibodies usually cannot be measured.If a person might have acute or primary HIV infection and is in the window period, a negative HIV ELISA and Western blot will not rule out HIV infection. More tests for HIV are needed.Veins and arteries vary in size from one patient to another, and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.Other risks associated with having blood drawn are slight, but may include:Excessive bleedingFainting or feeling light-headedHematoma (blood accumulating under the skin)Infection (a slight risk any time the skin is broken)People who are at high risk (men who have sex with men, injection drug users and their sexual partners, commercial sex workers) should be regularly tested for HIV.If the health care provider suspects early acute HIV infection, other tests (such as HIV viral load) will be needed to confirm this diagnosis, because the HIV ELISA/Western blot test may be negative during this window period.HIV testingDewar R, Goldstein D, Maldarelli F. Diagnosis of human immunodeficiency virus infection. In: Mandell GL, Bennett GE, Dolin R, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 7th ed. Philadelphia, Pa: Elsevier Churchill-L 2009:chap 119.Warner EA, Herold AH. Interpreting laboratory tests. In: Rakel RE, Rakel DP, eds. Textbook of family medicine. 8th ed. Philadelphia, Pa: Elsevier S 2011:chap 15Updated by: Jatin M. Vyas, MD, PhD, Assistant Professor in Medicine, Harvard Medical S Assistant in Medicine, Division of Infectious Disease, Department of Medicine, Massachusetts General Hospital. Also reviewed by David Zieve, MD, MHA, Bethanne Black, and the A.D.A.M. Editorial Team.MedlinePlus TopicsImagesRead MoreA.D.A.M., Inc. is accredited by URAC, also known as the American Accreditation HealthCare Commission (www.urac.org). URAC's
is an independent audit to verify that A.D.A.M. follows rigorous standards of quality and accountability. A.D.A.M. is among the first to achieve this important distinction for online health information and services. Learn more about A.D.A.M.'s ,
and . A.D.A.M. is also a founding member of Hi-Ethics and subscribes to the principles of the Health on the Net Foundation (www.hon.ch).The information provided herein should not be used during any medical emergency or for the diagnosis or treatment of any medical condition. A licensed physician should be consulted for diagnosis and treatment of any and all medical conditions. Call 911 for all medical emergencies. Links to other sites are provided for information only -- they do not constitute endorsements of those other sites. Copyright , A.D.A.M., Inc. Duplication for commercial use must be authorized in writing by ADAM Health Solutions.502 Bad Gateway
502 Bad Gatewaywestern blot是干什么的_百度作业帮
western blot是干什么的
Western Blot中文一般称为蛋白质印迹.它是分子生物学、生物化学和免疫遗传学中常用的一种实验方法.其基本原理是通过特异性抗体对凝胶电泳处理过的细胞或生物组织样品进行着色.通过分析着色的位置和着色深度获得特定蛋白质在所分析的细胞或组织中的表达情况的信息.Western Blot采用的是聚丙烯酰胺凝胶电泳,被检测物是蛋白质,“探针”是抗体,“显色”用标记的二抗.经过PAGE分离的蛋白质样品,转移到固相载体（例如硝酸纤维素薄膜）上,固相载体以非共价键形式吸附蛋白质,且能保持电泳分离的多肽类型及其生物学活性不变.以固相载体上的蛋白质或多肽作为抗原,与对应的抗体起免疫反应,再与酶或同位素标记的第二抗体起反应,经过底物显色或放射自显影以检测电泳分离的特异性目的基因表达的蛋白成分.Western Blot显色的方法主要有以下几种：　　i.放射自显影　　ii.底物化学发光ECL　　iii.底物荧光ECF　　iv.底物DAB呈色值得一提的是,western blot 这个名称的由来很有意思.最开始做印迹工作的是一个叫做Southern的科学家,但印迹的对象是DNA链,他把这种技术称为Southern blot,后来类似的出现了两个过程相似,但是对象不同的印迹方法,一个针对RNA,一个对蛋白质,人们分别把这两种技术的称为Northern和Western,与这两个技术的发明人没有关系了.
简单说，就是检测蛋白的
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