DNA-directed self-assembly of fluorescent dye-labeled streptavidin arrays for protein detection.
J Nanosci Nanotechnol. ):6048-51.
Affiliation
In this work, a DNA lattice was utilized to direct protein self-assembly. A model biosensor system was fabricated by self-assembly of rhodamine dye-labeled streptavidin molecules on a two-tile DNA system. This self-assembly nanofabrication technique has numerous possible biotechnology applications, including use in multiplex biosensing systems that have binding of several biological molecules encoded in a multiple-tile DNA lattice.
[PubMed - indexed for MEDLINE]
Citation 6 of 354
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Review articleFOSL1 controls the assembly of endothelial cells into capillary tubes by direct repression of αv and β3 integrin transcription.
1, , , , , , , , , , .
Mol Cell Biol. ):. doi: 10.1128/MCB.01054-12. Epub
2013 Jan 14.
Affiliation
To form three-dimensional capillary tubes, endothelial cells must establish contacts with the extracellular matrix that provides signals for their proliferation, migration, and differentiation. The transcription factor Fosl1 plays a key role in the vasculogenic and angiogenic processes as Fosl1 knockout embryos die with vascular defects in extraembryonic tissues. Here, we show that Fosl1(-/-) embryonic stem cells differentiate into endothelial cells but fail to correctly assemble into primitive capillaries and to form tube-like structures. FOSL1 silencing affects in vitro angiogenesis, increases cell adhesion, and decreases cell mobility of primary human endothelial cells (HUVEC). We further show that FOSL1 is a repressor of αv and β3 integrin expression and that the down-modulation of αvβ3 rescues the angiogenic phenotype in FOSL1-silenced HUVEC, while the ectopic expression of αvβ3 alone reproduces the phenotypic alterations induced by FOSL1 knockdown. FOSL1 represses the transcription of both αv and β3 integrin genes by binding together with JunD to their proximal promoter via the transcription factor SP1. These data suggest that FOSL1-dependent negative regulation of αvβ3 expression on endothelial cells is required for endothelial assembly into vessel structures.
[PubMed - indexed for MEDLINE]
PMCID PMC3592019
Citation 29 of 262962
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Review articleassembly是什么意思，装配，组装翻译-生物医药大词典
您现在的位置： >>
>> 词汇解释: assembly
装配，组装
分类：&&&&&&&相关的句子：&&&|&&&&&&&|&&&&
以下为句子列表：
英文: [NIV] Praise the Lord. Sing to the Lord a new song, his praise in the assembly of the saints. 中文:
1[和合]你们要赞美耶和华，向耶和华唱新1歌，在2圣民的会中赞美他。&&&&&&&
英文: [Perng,C., Y.C.Lai, Z.Y.Zhuang and Z.P.Ho (2006) The layout study of applying scheduling technique to job based assembly factory, Scheduling &VRP Section, The Third Conference on Operations Research of Taiwan, No.59, P.11:1-7, Yuan-Ze U., Zhong-Li City, T 中文:
彭泉、赖奕铨、庄政益、何子平(2006)运用排程技术于零工式订单组装工厂设施规划之研究，排程规划与车辆途程组，第三届作业研究学术研讨会论文集，11月18日，第59篇，P.11:1-7，元智大学，中坜市，桃园县，台湾。&&&&&&&
英文: [br]We are looking forward to sharing ideas regarding international real estate development during your upcoming meeting, [red]and if [/red]this meets with your approval, are planning for Beijing Assembly on **(date). 中文:
如果您同意我们这一决定并计划在2007年*月*日举行北京年会的话，我们期待与您在3月即将举行的**会议上就国际房地产的发展方面问题交流意见?&&&&&&&
英文: “Few would argue with the idea of recycling computers rather than depositing them in landfills or, as is sometimes the practice, shipping them off for disassembly by workers in poor regions of Asia. 中文:
意思是：比之将废旧计算机填埋在垃圾场或象通常那样运到亚洲贫困地区拆卸，很少人会不同意由厂家回收废旧计算机的计划。&&&&&&&
英文: “if the assembly line is redesigned, a smooth work flow can be achieved and time and energy can be saved. 中文:
“如果把装配线重新设计一下，生产流程就会达到平稳，并能节省时间和精力。”&&&&&&&
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由团队研发Assembly of desmosomal cadherins into desmosomes is isoform dependent.
1, , , , , .
J Invest Dermatol. ):26-35.
Affiliation
Desmosomes are intercellular adhesive junctions that exhibit cell- and differentiation-specific differences in their molecular composition. In complex epithelia, desmosomes contain multiple representatives of the desmosomal cadherin family, which includes three desmogleins and three desmocollins. Rules governing the assembly of desmosomal cadherin isoforms into desmosomes of different cell types are unknown. Here we compared the assembly properties of desmoglein 2 (Dsg2) and desmocollin 2 (Dsc2), which are widely expressed, with Dsg1 and Dsc1, which are expressed in the differentiated layers of complex epithelia, by introducing myc-tagged forms into simple and squamous epithelial cells that do not express Dsg1 or Dsc1. Dsc2.myc and Dsg2.myc assembled efficiently into desmosomes in every cell type in spite of significant shifts in the stoichiometric relationship between desmogleins and desmocollins. In contrast, Dsc1a.myc, Dsc1b.myc, and Dsg1.myc did not stably incorporate into desmosomes in any line. Coexpression of Dsc1a.myc or Dsc1b.myc and Dsg1.myc did not lead to their colocalization and failed to enhance incorporation of either cadherin into desmosomes. Dsg1.myc, but not Dsc1a, Dsc1b, disrupted desmosome assembly in a cell-type-specific manner, and disruption correlated with the recruitment of Dsg1.myc, but not Dsc1a or Dsc1b, into a Triton-insoluble pool. The plakoglobin:E-cadherin ratio decreased in Dsg1-expressing cells with disrupted desmosomes, but a decrease was also observed in a Dsc1a line. Thus, a modest reduction of plakoglobin associated with E-cadherin is apparently not sufficient to disrupt desmosome assembly. Our results demonstrate that desmosome assembly tolerates large shifts in cadherin stoichiometry, but is sensitive to isoform-specific differences exhibited by desmogleins and desmocollins.
[PubMed - indexed for MEDLINE]
Nature Publishing Group:
Citation 5 of 340
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